Please refer to the Transcriber's Notes at the end of this document.

PRESERVATION
OF BULL SEMEN

AT SUB-ZERO TEMPERATURES

Bulletin 621

UNIVERSITY OF ILLINOIS
AGRICULTURAL EXPERIMENT STATION

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Page
EARLY WORK ON FREEZING SEMEN		5

SELECTION OF SEMEN FOR FREEZING		6
	Predicting freezability	6
	Freezability of first and second ejaculates	7
	Freezability of several consecutive ejaculates	7
	Freezability of epididymal sperm	9
	Freezability of washed sperm	10

EXTENDERS FOR FREEZING BULL SEMEN		10
	Proportion of egg yolk in final diluent	10
	Citrate level in final diluent	11
	Storing and freezing diluent	12
	Other diluents	13

DILUTION RATES		14
	Effect of further dilution and refreezing	14

GLYCEROL ADDITIONS		17